Ethylbenzothiazoline
TīmeklisABTS (2,2′ -Azino-bis- (3-ethylbenzothiazoline-6-sulfonic acid), diammonium salt) is a very sensitive chromogenic peroxidase substrate widely used for ELISA applications. …
Ethylbenzothiazoline
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Tīmeklis2024. gada 10. apr. · A micro-technique of enzyme-linked immunosorbent assay (ELISA) using ABTS, as a substrate for HRP conjugate is studied. In a comparative study among 4 substrates, namely; 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT) and ABTS, for HRP in terms of sensitivity, ABTS is the most … Tīmeklis2024. gada 14. apr. · In this study, the methanol extract of Siegesbeckia orientalis L. was used for phytochemical analysis and investigation of the antioxidant capacity by three methods including 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS•+), 2,2-diphenyl-1-picrylhydrazyl (DPPH), total reducing power assay (PFRAP).
In biochemistry, ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) is a chemical compound used to observe the reaction kinetics of specific enzymes. A common use for it is in the enzyme-linked immunosorbent assay (ELISA) to detect the binding of molecules to each other. It is commonly used as a substrate with hydrogen peroxide for a peroxidase enzy… TīmeklisBiochem/physiol Actions. 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) is a cation and undergoes oxidation in the presence of hydrogen peroxide. …
TīmeklisA peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS).††† ABTS, 2,2′-Azino-bis(3 … Tīmeklis2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end …
Tīmeklis2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation (ABTS*+) is a stable free radical frequently used for estimating the total antioxidant capacity (TAC) of natural products. The existing methods for ABTS*+ radical-scavenging activity assays are diverse in pre-dilut …
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