WebE-Syt1 Antibody (A303-361A) in IP Detection of human E-Syt1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-E-Syt1 antibody A303-361A used for … WebMar 21, 2024 · SYT1 (Synaptotagmin 1) is a Protein Coding gene. Diseases associated with SYT1 include Baker-Gordon Syndrome and Syndromic Intellectual Disability . Among its …
SYT1-associated neurodevelopmental disorder: a case …
WebAnti-SYT1 antibody (ab254104) at 1/1000 dilution + Rat cortex lysate Predicted band size: 47 kDa. Protocols. To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on. Click here to view the general protocols. Datasheets and documents ... WebApr 27, 2024 · Mouse monoclonal anti-syt1 (synaptotagmin-1) antibody (mAb 48; Matthew et al., 1981) and anti-SV2 ( Buckley and Kelly, 1985) antibodies were purified by protein G … pa days york region district school board
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WebJun 3, 2024 · D, Quantification of the amount of HA-Syt1 co-immunoprecipitated (co-IP) was normalized to the amount of SV2A-mCer ±SEM (n = 7, Student's t test, **p = 0.0035). First, we determined whether the cytoplasmic loop of SV2A in which the R383Q mutation resides interacts with Syt1. WebAntibodies: Affinity purified rabbit anti-E-Syt1 antibody A303-362A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. E-Syt1 was also immunoprecipitated by rabbit anti-E-Syt1 antibody A303-361A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B). WebApr 18, 2016 · Syt1 bears a highly conserved phosphorylation site (threonine 112) in a putative α-helix in the linker between the transmembrane (TM) and C2A domain that can be phosphorylated by PKC and Ca 2+ /calmodulin (CaM)-dependent protein kinase II (CaMK-II, refs. 13 and 18 ). pa days halton region