Thermostable exoshells
WebbDeshpande S et al. Thermostable exoshells fold and stabilize recombinant proteins. Nat Commun 8:1442 (2024). PubMed: 29129910; Customer reviews and Q&As. Show All … WebbThermostable exoshell (tES) is a representative 12 nm NP with an 8 nm interior cavity. In this thesis, we show that the encapsulated enzymes remain highly stable in vitro. Local …
Thermostable exoshells
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WebbHere the authors use nanoscale exoshells to improve soluble yield, functional yield ... A general approach to protein folding using thermostable exoshells Samira Sadeghi, … Webb20 nov. 2024 · He is currently the primary investigator on a multi-institutional, 3,000-person observational trial in Singapore that studies how personalized drug metabolism affects drug response. The article can be found at: Deshpande et al. (2024) Thermostable Exoshells Fold and Stabilize Recombinant Proteins.
WebbA general approach to protein folding using thermostable exoshells Nature Communications 2024-09-29 Journal article DOI: 10.1038/s41467-021-25996-4 … WebbKEYWORDS: thermostable, exoshell, protein encapsulation, bioorthogonal catalysis, tumor regression C urrent treatment regimens for cancer are often toxic and carry the burden …
WebbThe expression and stabilization of recombinant proteins is fundamental to basic and applied biology. Here we have engineered a thermostable protein nanoparticle (tES) to … WebbWe report the use of self-assembling, porous exoshells (tESs) to encapsulate and deliver an iron-containing reaction center for the treatment of breast cancer. The catalytic center is paired with indole-3-acetic acid (IAA), a natural product found in edible plants, which undergoes oxidative decarboxylation, via reduction of iron(III) to iron(II), to produce free …
Webb29 sep. 2024 · A thermostable protein nanoparticle (tES) is engineered to improve both expression and stabilization of recombinant proteins using this technology, improving …
Webb29 sep. 2024 · In this conversation. Verified account Protected Tweets @; Suggested users the lion above the door planningFar-UV CD spectra (260–190 nm) were recorded using a Chirascan Circular Dichroism Spectrometer (Applied Photophysics). Protein samples (0.1 mg/mL) were dissolved in 10 mM phosphate buffer and measurements were carried out at room temperature using a 0.1-cm path length-stoppered cuvette. The heat-induced … Visa mer The pRSF1b expression vector (Merck) and pBAD/HisB vector (Life Technologies) were used for cloning. Chemically competent XL1 Blue E. coli cells (catalog #RH119-80; Simply Science) and BL21 (DE3) E. colicells … Visa mer The following reagents were used: restriction enzymes NcoI, EagI, and SpeI (New England BioLabs), Expresslink T4 DNA ligase (Life … Visa mer For the expression of each clone, a single positive colony was selected from a freshly transformed plate and grown in 100-mL LB broth, with kanamycin (50 µg/mL, for a protein gene on pRSF1b vector), ampicillin (100 … Visa mer AfFtn gene was selected based on the sequence in GenBank AF_RS04235. The gene with mutations for C-terminus truncation, as well as altered charges was synthesized from … Visa mer the lion a a sheep quicklyWebbWe report the use of self-assembling, porous exoshells (tESs) to encapsulate and deliver an iron-containing reaction center for the treatment of breast cancer. The catalytic … the lion 1 old hall st malpas sy14 8ne ukWebb24 apr. 2024 · Background More than 7000 papers related to “protein refolding” have been published to date, with approximately 300 reports each year during the last decade. … ticketmal studio lightWebb30 sep. 2024 · Thermolabile proteins internalized by tES are resistant to thermal, organic, chaotropic, and proteolytic denaturation and can be released from the tES assembly with … the lion 40kWebb13 nov. 2024 · To demonstrate the wide versatility of their technology, the researchers tested their exoshell technology by fusing one of the 24 ferritin subunits around three POIs with diverse properties --... the lion above the door reviewWebb1 juni 2008 · The use of nanoscale exoshells (tES) to provide complementary nanoenvironments for the folding and release of 12 highly diverse protein substrates ranging from small protein toxins to human albumin, a dimeric protein (alkaline phosphatase), a trimeric ion channel (Omp2a) and the tetrameric tumor suppressor, p53 … the lion account